Membrane potential measurement in parotid acinar cells
نویسندگان
چکیده
منابع مشابه
Rat Parotid Acinar Cells
We used the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6')-carboxyfluorescein to monitor the recovery of the intracellular pH (pH,) of rat parotid acini from an NH+-induced alkaline load. This recovery was markedly inhibited by the loop diuretic bumetanide and by Clremoval, indicating that it is largely due to NH' entry via the basolateral Na'-K+2Clcotransporter. The rate of recov...
متن کاملCapacitative calcium entry in parotid acinar cells.
The intracellular Ca2+ indicator, fura-2, was used to monitor changes in cytosolic [Ca2+] in parotid acinar cells. When parotid cells were incubated in a medium containing low [Ca2+], and [Ca2+] was restored to the physiological range, there was a small increase in cytosolic [Ca2+]. If, however, the cells were first activated by a muscarinic agonist, and receptor activation was terminated befor...
متن کاملHydrogen peroxide inhibits Ca2+ efflux through plasma membrane Ca2+-ATPase in mouse parotid acinar cells
Intracellular Ca2+ mobilization is closely linked with the initiation of salivary secretion in parotid acinar cells. Reactive oxygen species (ROS) are known to be related to a variety of oxidative stress-induced cellular disorders and believed to be involved in salivary impairments. In this study, we investigated the underlying mechanism of hydrogen peroxide (H2O2) on cytosolic Ca2+ accumulatio...
متن کاملApical Ca2+-activated potassium channels in mouse parotid acinar cells
Ca(2+) activation of Cl and K channels is a key event underlying stimulated fluid secretion from parotid salivary glands. Cl channels are exclusively present on the apical plasma membrane (PM), whereas the localization of K channels has not been established. Mathematical models have suggested that localization of some K channels to the apical PM is optimum for fluid secretion. A combination of ...
متن کاملApical Ca-activated potassium channels in mouse parotid acinar cells
The major physiological function of parotid acinar cells is the production of saliva, a watery fluid containing electrolytes and a complex mixture of proteins (Cook et al., 1994; Melvin et al., 2005). The driving force for fluid and electrolyte secretion is the vectoral, trans-epithelial movement of Cl. Cl is accumulated intracellularly via the concerted effort of several transporters, and afte...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1973
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1973.sp010342